<p>Although only a few genes were statistically significantly differentially expressed in ALI-cultured AECs in response to flagellin, we evaluated two top candidates (CCL20, ICAM1) using qPCR. However, note that even these two candidate genes were more significantly up-regulated following flagellin exposure in the monolayer culture system compared to ALI culture. For each condition, mean value ± standard deviation is shown.</p
<p>For in vitro detection of bacterial mRNA, <i>Salmonella</i> (BRD509) were cultured under SPI1-ind...
<p>(<b>A</b>) The relative abundance of <i>fliF</i>, <i>flgE</i>, <i>flbT</i>, and <i>fliC</i> mRNAs...
Animals and plants carry recognition systems to sense bacterial flagellin. Flagellin perception in A...
<p>Three highly up-regulated genes in response to flagellin in monolayer-cultured AECs (NOS2, CCL2, ...
<p>Note the widespread transcriptional response of AECs to flagellin in monolayer cultures compared ...
<p>Note the very large number of differentially expressed genes between ALI vs. monolayer AEC cultur...
<p>In this analysis, the contributions of: (i) culture methodology (monolayer, ALI); (ii) whole-geno...
<p>We used ELISA to assess secreted protein levels of (A) CCL20 and (B) IL8 at baseline and followin...
Airway epithelial cells (AEC) are critical components of the inflammatory and immune response during...
<p>Bars indicate the relative activity of a luciferase reporter gene under the control of an NF-κB d...
<p>Each strain (ca. 10<sup>8</sup> cfu/ml) was grown in nutrient-poor FSLW and incubated overnight s...
<div><p>Airway epithelial cells (AEC) are critical components of the inflammatory and immune respons...
<p>Data from qPCR are expressed as fold variation of gene expression in spheroids normalized <i>vs</...
<div><p>(A) Western blot analysis of FlaA levels in cellular fractions of wild-type and Δ<i>mogR</i>...
<p>Relative expression levels obtained through qRT-PCR were compared with microarray expression leve...
<p>For in vitro detection of bacterial mRNA, <i>Salmonella</i> (BRD509) were cultured under SPI1-ind...
<p>(<b>A</b>) The relative abundance of <i>fliF</i>, <i>flgE</i>, <i>flbT</i>, and <i>fliC</i> mRNAs...
Animals and plants carry recognition systems to sense bacterial flagellin. Flagellin perception in A...
<p>Three highly up-regulated genes in response to flagellin in monolayer-cultured AECs (NOS2, CCL2, ...
<p>Note the widespread transcriptional response of AECs to flagellin in monolayer cultures compared ...
<p>Note the very large number of differentially expressed genes between ALI vs. monolayer AEC cultur...
<p>In this analysis, the contributions of: (i) culture methodology (monolayer, ALI); (ii) whole-geno...
<p>We used ELISA to assess secreted protein levels of (A) CCL20 and (B) IL8 at baseline and followin...
Airway epithelial cells (AEC) are critical components of the inflammatory and immune response during...
<p>Bars indicate the relative activity of a luciferase reporter gene under the control of an NF-κB d...
<p>Each strain (ca. 10<sup>8</sup> cfu/ml) was grown in nutrient-poor FSLW and incubated overnight s...
<div><p>Airway epithelial cells (AEC) are critical components of the inflammatory and immune respons...
<p>Data from qPCR are expressed as fold variation of gene expression in spheroids normalized <i>vs</...
<div><p>(A) Western blot analysis of FlaA levels in cellular fractions of wild-type and Δ<i>mogR</i>...
<p>Relative expression levels obtained through qRT-PCR were compared with microarray expression leve...
<p>For in vitro detection of bacterial mRNA, <i>Salmonella</i> (BRD509) were cultured under SPI1-ind...
<p>(<b>A</b>) The relative abundance of <i>fliF</i>, <i>flgE</i>, <i>flbT</i>, and <i>fliC</i> mRNAs...
Animals and plants carry recognition systems to sense bacterial flagellin. Flagellin perception in A...