Rickettsial gene-like sequences possibly located in the nuclear genomes of <i>Volvox carteri</i> strains.

<p>Schematic representations of arrangements/synteny (A-D) in, and semi-quantitative genomic PCR data (E) from, several rickettsial gene-like sequences possibly located in the nuclear genomes of <i>V</i>. <i>carteri</i> strains. Coding DNA sequence (CDS)-like regions are shown as boxes. Rickettsial CDS-like regions are shown in pale yellow, the <i>V</i>. <i>carteri</i> transposon <i>Jordan</i>-like region in green and others in black. Placement of boxes above/below the line indicates the gene direction (from left-to-right or right-to-left, respectively). Black double-headed arrows on the baseline indicate the regions sequenced in the present study. Colored triangles under boxes indicate the primers used for semi-quantitative genomic PCR (16S rRNA gene 5′-region: magenta, 16S rRNA gene 3′-region: light blue, <i>murB</i>: orange, <i>ddlB</i>: green; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116192#pone.0116192.g002" target="_blank">Fig. 2E</a>). For accession numbers of sequences used in this figure, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116192#pone.0116192.s010" target="_blank">S3 Table</a>. (A) Sequences including rickettsial CDS-like regions of <i>V</i>. <i>carteri</i> f. <i>nagariensis</i> strains UTEX 1886, NIES-397 and NIES-398. (B) Sequences including rickettsial gene homologs of <i>V</i>. <i>carteri</i> f. <i>weismannia</i> strains UTEX 1875 and UTEX 1876. Plus (+) indicates a frameshift deletion. (C) Sequence including rickettsial <i>murB</i>-like sequence of <i>V</i>. <i>carteri</i> f. <i>weismannia</i> strain UTEX 2170. (D) Sequences including rickettsial 16S rRNA gene-like sequences of <i>V</i>. <i>carteri</i> f. <i>kawasakiensis</i> NIES-732 and NIES-733. (E) Semi-quantitative genomic PCR of rickettsial genes and gene-like sequences. Each rickettsial gene-like sequence was amplified via genomic PCR using rickettsia-specific primer sets (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116192#sec013" target="_blank">Materials and Methods</a>). The positions of primer sets with reference to target positions are shown in both <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116192#pone.0116192.g001" target="_blank">Fig. 1</a> and this <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116192#pone.0116192.g002" target="_blank">Fig. 2</a>. As a control, the actin gene was amplified. <i>Chlamydomonas reinhardtii</i> strain CC-503 was used as negative control.