<i>S</i>-methyl DM1 does not induce oligomerization of ECFP tubulin in ECFP-PTK2 cells.

<p>A. Intracellular fluorescence autocorrelation curves for ECFP tubulin in ECFP-PTK2 cells treated with various microtubule depolymerizing agents. Each curve was the average of individual cell measurements: a total of 30 cells and 144 measurements were collected for average for non-treated cells, a total of 12 cells and 60 measurements for nocodazole, a total of 9 cells and 54 measurements for both <i>S</i>-methyl DM1 and demecolcine, and a total of 11 cells and 52 measurements for vinblastine. B. Diffusion coefficients of cytoplasmic tubulin obtained from the FCS measurements in living cells treated with microtubule depolymerizing agents. Whiskers indicate minimum to maximum and box median value (line within the box).