Long term immune responses, phenotype and secondary in vivo expansion.

<p>Mice were either infected with LCMV clone 13 or Ad-IiGP or Ad-NP vaccinated and then challenged with LCMV clone 13 as indicated. One year after LCMV clone 13 infection, mice were sacrificed and spleens were harvested for characterization of the CD8+ T cell response. <b>a-b</b>) Enumeration of CD8+ T cells responding to specific GP (<b>a</b>) or NP (<b>b</b>) peptide stimulation with interferon-γ production. <b>c-d</b>) Fraction of CD8+ T cells from Ad-IiGP (<b>c</b>) and Ad-NP (<b>d</b>) vaccinated mice responding to individual specific peptides as a fraction of total numbers of GP plus NP specific cells. The small difference in the sizes of the pie charts reflects the difference in the averages of total numbers of cells recognizing GP and NP epitopes in the two groups. <b>e-g</b>) phenotype of responding cells. The percentage of cell producing interferon-γ in response to GP33 (<b>e</b>), GP276 (<b>f</b>) or NP396 (<b>g</b>) peptide, that also expressed certain combinations of CD127 and KLRG-1 are depicted, CD127+/KLRG1+ (DP), CD127+/KLRG1- (MPEC) and CD127-/KLRG1+ (SLEC)). Shown are mean + SEM for 8 animals per group. <b>h</b>) Expansion following vaccinia GP or vaccinia NP challenge. 10.000 GP33+GP34+GP276 or 10.000 NP396 specific cells pooled from the animals described above (CD45.2+) were transferred to naïve recipients (CD45.1+), and these were challenged the following day with vaccinia expressing NP (NP396) or GP (GP33, GP34, GP276 and GP pool). Fold expansion is the number of donor T cells quantified by the indicated tetramer in vaccinia infected recipients as compared to the number detectable in unchallenged recipients. Shown are mean +SEM of 5 recipient mice per group.