TRPM3-dependent intracellular calcium increases in mouse retinal ganglion cells.

<p>Ca²⁺ signals were recorded from immunopanned ganglion cells by Fura-2-based ratiometric imaging. Dotted lines indicate where gaps were introduced in some traces to facilitate proper alignment. Greater than 50% of ganglion cells from WT mice responded to Pregnenolone Sulfate (PS)-activated rises in calcium (<b>B</b>) compared to baseline (<b>A</b>); the PS signal was abolished by pretreatment with mefenamic acid (MA; <b>C</b>). In the TRPM3^-/- mice, no cells produced a large response to PS; a few cells, however, gave a small response that was not blocked by MA. At the end of the experiment, cells were depolarized with KCl perfusion to verify viability (<b>D</b>).