HuR only binds to the Bcl2 ARE-rich sequence after B cell activation.

<p><b>A</b>, Genomic annotation of HuR- crosslinks across the Bcl2 locus. Representative data from iCLIP experiments (n = 3) performed with total cells extracts from freshly isolated B cells or LPS-activated B cells are shown. The number on the left hand of each genomic track refers to the maximum number of unique counts identified at a single nucleotide level (called cross(x)-links). Bcl2 expression data from CD19⁺ CD3^- splenic B cells (RNAseq from ImmGen.org) and sequence homology analysis across 30 species are shown in independent genomic tracks. <b>B</b>, Representation of HuR- crosslinks within the 3’UTR of Bcl2. <b>C</b>, Analysis of HuR binding to the Bcl2 ARE-rich sequence. <b>D</b>, Sequence analysis of HuR binding to the ARE. Single nucleotide interactions with HuR are coloured in blue.