Screening of the peptide pools comprising ASP-2 amino acids 261–380.

<p>BALB/c mice were immunized with two doses of the αDEC-ASP2 or Iso-ASP2 mAbs as described in material and methods. Fifteen days after the administration of the second dose, mice were euthanized and their splenocytes were plated in the presence or absence of the three peptide pools (5 μg/mL) shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117778#pone.0117778.t001" target="_blank">Table 1</a>. The number of IFN-γ producing cells was detected by ELISPOT and the bars represent the mean ± SD of pooled animals performed in triplicates. Numbers of IFN-γ producing cells in the absence of any stimulus were subtracted from the stimulated samples. Experiment was analyzed by one-way ANOVA followed by the post-test HSD Tukey. * refers to p<0.05. The graph is representative of 2 experiments.