<p>5′-fluorescein-labeled d- or l-RNA substrate (200 nM) was incubated with (A) d- or l-hammerhead ribozyme (2 μM) or (B) d- or l-DNAzyme (2 μM) in 50 mM Tris (pH 7.5), 10 mM MgCl<sub>2</sub> for 5 h at 37°C. RNA substrate and its 5′-hydrolysis products were visualized via a 5′-fluorescein tag. Ribozymes and DNAzymes were stained with ethidium bromide. Data is representative of three independent experiments.</p
88 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.This thesis research uses sing...
Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficie...
With the discovery of small non-coding RNA (ncRNA) molecules as regulators for cellular processes, i...
<p>l-hammerhead ribozyme solution (2 μM) was artificially contaminated with enantiomeric d-ribozyme ...
Catalytic nucleic acids are regarded as potential therapeutic agents and biosensors. The catalytic a...
<p>Reactions were carried out at 37°C in a 50 mM Tris-HCl, pH 7.5, buffer, containing 10 mM MgCl<sub...
Using fluorescence microscopy as a tool to study biological systems has been proven to be useful in ...
This thesis reports the results of experiments designed to help elucidate the catalytic mechanisms o...
<p>Lanes 1 and 2: D- and L-RNA targets (0.2 µM) incubated in 50 mM Tris-HCl buffer, pH 7.5, and 10 m...
<p>Left panel: Target L-DNA1 in buffer without MgCl<sub>2</sub> (C), the same with 25 mM of MgCl<sub...
<p><b>(A)</b> Schematic illustrations of the sequences and secondary structures of hammerhead ribozy...
In this manuscript we describe for the first time mirror image catalytic nucleic acids (Spiegelzymes...
A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and molecular...
<div><p>A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and m...
The RNA World hypothesis suggests prebiotic enzymes were RNA molecules (ribozymes) rather than prote...
88 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.This thesis research uses sing...
Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficie...
With the discovery of small non-coding RNA (ncRNA) molecules as regulators for cellular processes, i...
<p>l-hammerhead ribozyme solution (2 μM) was artificially contaminated with enantiomeric d-ribozyme ...
Catalytic nucleic acids are regarded as potential therapeutic agents and biosensors. The catalytic a...
<p>Reactions were carried out at 37°C in a 50 mM Tris-HCl, pH 7.5, buffer, containing 10 mM MgCl<sub...
Using fluorescence microscopy as a tool to study biological systems has been proven to be useful in ...
This thesis reports the results of experiments designed to help elucidate the catalytic mechanisms o...
<p>Lanes 1 and 2: D- and L-RNA targets (0.2 µM) incubated in 50 mM Tris-HCl buffer, pH 7.5, and 10 m...
<p>Left panel: Target L-DNA1 in buffer without MgCl<sub>2</sub> (C), the same with 25 mM of MgCl<sub...
<p><b>(A)</b> Schematic illustrations of the sequences and secondary structures of hammerhead ribozy...
In this manuscript we describe for the first time mirror image catalytic nucleic acids (Spiegelzymes...
A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and molecular...
<div><p>A major challenge for the application of RNA- or DNA-oligonucleotides in biotechnology and m...
The RNA World hypothesis suggests prebiotic enzymes were RNA molecules (ribozymes) rather than prote...
88 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002.This thesis research uses sing...
Through evolution, enzymes have developed subtle modes of activation in order to ensure the sufficie...
With the discovery of small non-coding RNA (ncRNA) molecules as regulators for cellular processes, i...