Quantitative real-time PCR (qPCR) validations of gene expression levels from digital gene expression (DGE) analysis.

<p>The qPCR values are presented as the averages of three independent experiments. The genes were randomly selected. “S3/H3” indicates a comparison between the expressions of the corresponding genes in the S3 library with that in the H3 library. The “S12/H12” and “S24/H24” ratios indicate analogous comparisons. The y-axis indicates the fold-changes obtained by two methods. The “A-F” indicates the genes with the assay names of Bra041014, Bra000150, Bra020731, Bra011843, Bra023573 and Bra000090, respectively.