Survival curve of indomethacin-treated zebrafish larvae after LPS immersion.

<p><b><i>Panel A</i>:</b> A scheme of zebrafish sepsis model. Zebrafish larvae were maintained in Ringer’s solution containing 2.5 μM indomethacin or 0.01% DMSO from 3 to 8 days after fertilization (dpf). Solution and dead larvae were washed and fresh medium was added daily. After 5 days of indomethacin treatment, solution was changed and the larvae were immersed in 75 μg/ml LPS. <b><i>Panel B</i>:</b> Survival curve of indomethacin treated zebrafish larvae in sepsis model. Data represents two independent experiments (n = 20). Statistical analysis was performed by Log-rank (Mantel-Cox) test between LPS Vs inhibitor+LPS groups. ***<i>P</i><0.0001.