The distribution of <i>E. coli</i> in tumors.

<p>(A)Tumor sections obtained on day 8 from mice injected with PBS, <i>E. coli</i> (lux/βG) or <i>E. coli</i> (lux) were stained with DAPI (blue), mouse anti-<i>E. coli</i> beta-glucuronidase antibody (green) and goat anti-<i>E. coli</i> antibody (red). Arrows indicate the location of <i>E. coli</i>. Dotted lines indicate the approximate border between necrotic areas and live cells. N, necrotic area. Bars, 50 μm. (B) The percentage of <i>E. coli</i> in the necrotic area of tumor tissue sections. n = 4. n.s., no significant difference.