The effect of L-arginine-HCl solution concentration on the induction of acute pancreatitis in FVB/n mice.

<p>Animals were treated with PS or 2×4 g/kg L-arginine-HCl i.p. as described in the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117588#pone.0117588.g001" target="_blank">Fig. 1</a>. The number of total / euthanized / sacrificed non-AP / sacrificed AP mice (based on histological analysis) were: 8 / 0 / 8 / 0 in the PS group, 12 / 0 / 1 / 11 in the 5% L-arginine-treated group, 12 / 8 / 1 / 3 in the 10% L-arginine-treated group. The euthanized animals and those that did not show any signs of AP according to histological analysis (sacrificed non-AP mice) were excluded from the measurements. <b>A</b>. The effectivity rate of L-arginine-induced AP in FVB/n mouse strain. <b>B</b>. Serum amylase activities, <b>C</b>. pancreatic MPO activities, and <b>D</b>. pancreatic IL-1β levels were determined from the control group and animals with AP. The histological pictures show representative H&E staining of pancreatic samples from mice treated i.p. with <b>E</b>. PS, <b>F</b>. 5% L-arginine, and <b>G</b>. 10% L-arginine. Scale bar = 100 μm. p<0.05 vs the *: control group or the #: 5% L-arginine-treated group.