ERK5 phosphorylation at Thr732 via ERK1/2 is required for nuclear localization in HEK293 cells.

<p><b>(A)</b> HEK293 cells were transfected with empty vector, mycERK5 wild-type (WT), mycER–32A or mycER–32E. The myc-tagged ERKs were stained with myc primary antibody and Alexa488-conjugated secondary antibody with Hoechst 33258. Scale bar = 25 μm. The ratio of nuclear localization was also examined. Values represent the means ± SEM. * <i>P</i> < 0.05 compared with WT (n = 3). <b>(B)</b> HEK293 cells were co-transfected with mycERK5-WT or mycERK5-KD and empty vector or RASV12. The myc-tagged ERKs were stained with myc primary antibody and Alexa488-conjugated secondary antibody with Hoechst 33258. Scale bar = 25 μm. The ratio of nuclear localization was also examined. Values represent the means ± SEM. *<i>P</i> < 0.05 compared with empty vector (n = 3).