Single clone binding analysis.

<p>Expression-normalized median fluorescence intensity (MFI) is plotted against logarithmic Ab concentrations for d-SOSIP (gray) and clones 4.3.B01 (black) and 4.3.D01 (green) selected after DNA shuffling. Binding isotherms to a human IgG1k control Ab are included as reference. Error bars represent standard deviations of triplicate measurements.