Expression of <i>C</i>. <i>albicans</i> environmentally responsive genes during invasive infection.

<p>Changes in expression levels during mouse kidney invasion for 248 <i>C</i>. <i>albicans</i> genes (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002076#pbio.1002076.s001" target="_blank">S1 Data</a>) are presented in a heat map format. Mean values of biological triplicates are shown for up-regulation (yellow) and down-regulation (blue) of genes at 12, 24, and 48 hr postinfection relative to mean inoculum levels (0 hr). Color saturation represents the extent of the expression change, with full saturation at 10-fold up- or down-regulation. (All heat maps in this article follow the same color scale.) Portions of the heat map are expanded to illustrate representative early up-regulated genes (top), late genes (middle), and early down-regulated genes (bottom). In these portions, individual samples are presented separately to illustrate reproducibility. We define early expression changes as significant differences between the inoculum and 12 hr sample. We define late expression changes as significant differences between the 12 and 48 hr samples. Significance refers to changes of ≥2-fold and a <i>p</i>-value < 0.05. The data for each sample were normalized to RNA levels from control gene <i>TDH3</i> before mean values were calculated. Our assignment criteria allow some dynamically regulated genes to fall into both the early and late expression classes. All numerical data for this figure are in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002076#pbio.1002076.s007" target="_blank">S7 Data</a>.