Binding capacity of mAbs HB0116 and HB0478 against with gt-C and gt-A HBsAg and the G145R variant.

<p><b>(A)</b> Binding of mAbs HB0116 and HB0478 to synthetic peptides covering the first external loop of small-HBsAg was demonstrated by ELISA. The sequences of the recombinant peptides used in the analysis are shown above: amino acids which vary between genotype C (gt-C) and genotype A (gt-A) are indicated in bold. The absorbance at 405 nm is shown on the Y axis. Average data of three independent experiments are shown. <b>(B)</b> The gt-C, gt-A, and G145R virions were immunoprecipitated with HB0116 or HB0478 and HBsAg in the precipitates was detected by Western blotting. Recombinant HBsAg protein was used as the positive control (P lane). Representative data of three independent experiments are shown.