MethPrimers amplification efficiency is affected by DNA methylation.

<p>a) <i>Myogenin</i> and b) <i>PSEN1</i> overall non-CpG methylation. c) and d) Amplification efficiency of primers on samples with differential overall non-CpG methylation was assessed by Real-Time PCR assay using MIPs (light grey columns) or MethPrimers (dark grey columns) for <i>myogenin</i> (c) and <i>PSEN1</i> (d). Bisulphite-treated PCR products and untreated PCR products were used as control of amplification efficiency. Values are expressed as fold n° versus control (C2C12 in 1% 24 hours for <i>myogenin</i>, SK-N-BE in complete medium for <i>PSEN1 used as respective calibrators</i>); <i>myogenin</i> amplification values were normalized using <i>PSEN1</i> PCR product amplification (added to mouse samples and used as exogenous reference) whereas <i>PSEN1</i> amplification values were normalized to <i>myogenin</i> PCR product amplification (in this case added to human samples and used as exogenous reference). *: <i>p</i><0.001 vs. ctrl.