UGT1A activity affects β-lap -induced apoptosis to Colon Cancer Cells.

<p>Cells were pretreated with propofol (100 μM) for 1 h or UGT1A siRNA for 24 h. Then cells were exposed to β-lap (5 μM) for 24 h and collected. Cell apoptosis was assessed by TUNEL assay. (A) Cell apoptosis in HCT116 cells with propofol/NAC/catalase pretreatment; (B) Cell apoptosis in HT29 cells with propofol/NAC/catalase pretreatment; (C) Cell apoptosis in HT29 cells with siRNA/NAC/catalase pretreatment. Results are presented as mean ± 3 SEM of at least three independent experiments (*P<0.05, **P<0.01, β-lap treatment vs. control cells; #P<0.05, NAC pretreatment vs. corresponding β-lap only; $P<0.05, catalase pretreatment vs. corresponding β-lap only).