ZII expression in the cerebellum of the western diamondback rattlesnake.

<p><b>A-D</b> shows photomicrographs of ZII (red) expression in serial coronal sections of the rattlesnake cerebellum (caudal to rostral). The dashed vertical lines indicate the midline. All Purkinje cells are ZII+. <b>E-G</b> shows a portion of the section in <b>D</b> (see inset) at higher magnification labeled for both ZII (red; <b>E</b>) and calbindin (CB; green; <b>F</b>). As seen in the overlay (<b>G</b>), all Purkinje cells are double-labeled. Similarly <b>H-J</b> shows a portion of the section in <b>C</b> at higher magnification labeled for both ZII and CB. <b>K</b> shows a dorsal view of the wholemount of the <i>C</i>. <i>atrox</i> brain that was stained for ZII. Note that the cerebellum is entirely ZII immunopositive. <b>L-N</b> shows a coronal section from a pigeon cerebellum processed for ZII (red; <b>L</b>) and CB (green; <b>M</b>). The overlay is shown in <b>N</b>. Other abbreviations; a = anterior, p = posterior, d = dorsal, v = ventral, gl = granule layer, PL = Purkinje Layer; ml = molecular layer. Scale bars: 250 μm in <b>A</b> (applies for <b>A-D</b>); 50 μm in <b>E</b> (applies for <b>E-G</b>), <b>H</b> (applies for <b>H-J</b>) and <b>L</b> (applies for <b>L-N</b>); 1mm in <b>K</b>.