Abnormal chromosome segregation and spindle assembly in <i>Ago2</i>^ADH oocytes.

<p>Chromosome and spindle dynamics in oocytes expressing AURKA-EGFP (green) and H2B-mCherry (red) were observed by time-lapse live confocal microscopy. Frames at the indicated stages of meiotic maturation were selected from the original time series (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005013#pgen.1005013.s014" target="_blank">S1</a>–<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005013#pgen.1005013.s015" target="_blank">S2</a> Movies), in which images were acquired every 18 min for 16 h. All images are maximal intensity projections of a confocal z series. A-F: <i>Ago2</i>^fl/ADH oocytes; G-L: <i>Ago2</i>^ADH oocytes. GV: germinal vesicle intact (A, G), GVBD: germinal vesicle breakdown (B, H), MI: metaphase I (C, I), AI: anaphase I (D, J), TI: telophase I (E, K), MII: metaphase II (F, L). The experiment was performed 3 times using at least 10 oocytes per group. Representative images are shown.