Dysregulation of adipogenic signaling in differentiating Plin1-/- SVCs.

<p>The SVC cells isolated from adipose tissues in Plin1+/+, Plin1+/-, and Plin1-/- mice were differentiated. (A) Relative mRNA expression of adipogenic genes at day 5 of differentiation.*, P < 0.05 compared with Plin1+/+ cells. (B) Immunoblotting. Protein expression of adipogenic modulators at day 5 of differentiation in the SVCs from epididymal (Epi) and inguinal (Ing) fat depots in Plin1+/+, Plin1+/-, and Plin1-/- mice. (C) Immunoblotting. Protein expression of adipogenic modulators in the SVCs differentiated for 0, 1, and 5 days. (D) Immunofluorescence of Plin2 and Plin1 switch on lipid droplets. Plin1-/- and Plin1+/+ SVCs were differentiated for 0, 1, 2, and 5 days and immunostained with primary antibody against Plin1 (upper 2 rows) and Plin2 (lower 2 rows) and FITC-conjugated lgG. Nuclei (blue) were stained with Hoechst 33258. Triangles at the central of lipid droplets denote the large droplets disassociated from Plin2 in a fully-differentiated Plin1+/+ adipocyte, compared to the smaller droplets associated with Plin2 in ill-differentiated Plin1-/- SVCs.