Exogenous Plin1 expression restores Plin1-/- adipocyte differentiation.

<p>Plin1-/- and Plin+/+ epididymal SVCs were infected with the adenovirus dually expressing green fluorescence protein (GFP) and Plin1 (Ad-Plin1, a−c), with the adenovirus expressing GFP and Plin2 (Ad-Plin2, d−f) as the control. Fluorescence microscopy of cells induced to differentiate into adipocytes for 4 days, then stained with Nile red. Lipid droplets exhibit yellow/gold fluorescence. GFP-positive cells express ectopic Plin1 or Plin2. Well-differentiated Plin1-/- cells with Ad-Plin1 infection (circles with solid line) and poorly-differentiated cells without Ad-Plin1 infection (circles with dashed line) are labeled in the field of vision (b, c).