Plin1 defect attenuates the SVC differentiation into adipocytes.

<p>(A) Effect of Plin1 gene dosage on adipocyte differentiation. SVCs were isolated from epididymal fat tissue in Plin1+/+, heterozygous Plin1+/-, and Plin1-/- mice, epididymal fat tissue were differentiated into adipocytes. At day 5, intracellular lipid droplets were stained with Oil-red O (red bright-field) and Nile red (red fluorescence). Nuclei (blue) were stained with Hoechst 33258. (B) Dynamic changes of the lipid droplets (red) in Plin1-/- and Plin1+/+ SVCs at day 0, 0.5, 1, 2, 3 and 4 of differentiation.