Cytosolic markers of ER associated stress and ER associated degradation (ERAD) in mutant T4R <i>RHO</i> and <i>WT</i> retinas 6 hours after light exposure.

<p><b>(A)</b> Differential expression of genes <i>VCP</i>, <i>HRD1</i>, <i>DNAJA1</i>, <i>DNAJB1</i>, <i>HSP70</i>, <i>HSP90AA1</i>, <i>HSP90AB1</i>, <i>and CASP12</i> in the retinas of three RHO ^T4R/T4R mutant dogs following light exposure. Displayed are the mean fold change (FC) differences compared to the contralateral shielded retinas; error bars represent the FC range (FC min to FC max) (*: p < 0.05). (<b>B)</b> immunoblots showing the protein levels of HSP90 and HSP70 in light exposed (E) compared to shielded (S) retinas of mutant (<i>RHO</i>^T4R/T4R, and <i>RHO</i>^T4R/+), and <i>WT</i> (+/+) dogs. A single retina from a wild-type dog kept under standard ambient kennel illumination (K) was included as a control of basal levels of HSP90 and HSP70. There are no changes in HSP90 and HSP70 protein levels associated with light exposure. Note: the HSP90 antibody used recognizes the products of both the HSP90AA1 and HSP90AB1 genes.