FHL1 reduces fibrosis and fat deposition in dystrophic <i>FRG1</i> mice.

<p>Representative images of transverse sections of (A) quadriceps and (B) trapezius muscle from 12-week-old wild type, <i>FRG1</i> and <i>FRG1</i>/<i>FHL1</i> mice stained with Masson’s trichrome to detect fibrosis within muscle. The percentage area of fibrosis staining in muscle was quantified from wild type (n = 4–6), <i>FRG1</i> (n = 4–6) and <i>FRG1</i>/<i>FHL1</i> (n = 5–6) mice. Representative images of transverse muscle sections from the (C) quadriceps and (D) trapezius muscle stained with Oil Red O to detect fat deposits within muscle. The percentage area of fat deposition in muscle was quantified in wild type (n = 3–4), <i>FRG1</i> (n = 4) and <i>FRG1</i>/<i>FHL1</i> (n = 4) mice. Data represent the mean ± SEM; *p&lt;0.05; **p&lt;0.005; ***p&lt;0.0005 determined by two-tailed Student’s T-test. Scale bars = 100μm.