Representative progress curves from multiplex assay screening <i>Pf</i>A-M1 and –M17 against the MMV400 library.

<p>The multiplex assay screened one compound (100 μM) against three enzymes. The activity of <i>Pf</i>A-M1 and—M17 were measured by change in fluorescence units over time. No inhibitor controls (n = 8) for <i>Pf</i>MAP activity were included in each plate screened (shown in orange). A representative of the ‘drug-like’ compounds (MMV403679) is indicated by asterisk (*). A ‘probe-like’ compound (MMV666023) by solid dots (•). The inhibitor control (Bestatin) is shown as a solid black line.