Western blot analysis of whole cell lysates of the <i>ΔflaB2</i><sub><i>S2</i></sub> strain complemented with <i>flaB2</i><sub><i>S2</i></sub> with mutations at various <i>N</i>-glycosylation sites.
- Yan Ding (39795)
- Kaoru Uchida (184405)
- Shin-Ichi Aizawa (184410)
- Kathleen Murphy (694108)
- Alison Berezuk (694109)
- Cezar M. Khursigara (694110)
- James P. J. Chong (22205)
- Ken F. Jarrell (503335)
Publication date
February 2015
DOIAbstract
<p>Mutant FlaB2<sub>S2</sub> proteins missing single to quadruple <i>N</i>-glycosylation sites showed smaller apparent molecular mass than that of wildtype FlaB2<sub>S2</sub>. The G6 which has extra glycosylation sequons migrated slower than wildtype FlaB2<sub>S2</sub>. The expression of G10 was not detectable on this blot with normal exposure time. 900: wildtype <i>M. maripaludis</i> S2 <i>Δhpt</i>. WT: <i>ΔflaB2</i><sub><i>S2</i></sub> complemented with wildtype <i>flaB2</i><sub><i>S2</i></sub>.</p
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