Effect of TNF concentration on neutrophil adhesion and migration through EC and into collagen gels.

<p>HUVEC were stimulated with 0, 1, 10 or 100U/ml TNF for 4 h. Neutrophils were added and allowed to adhere for 10 min before non-adherent cells were washed off and data collected at 15 min, 1 h, 3 h and 24 h. A. Total adherent neutrophils after 15min (% of those added). B. Neutrophils that were transmigrated across the EC (% of those added). C. Neutrophils that were adherent on top of the endothelial monolayer (% of those added). D. Neutrophils that had transmigrated across the EC but were just under the monolayer (% of those added). E. Neutrophils that had migrated into the gel (% of those added). F. Average depth penetrated by the neutrophils that had transmigrated. In A and B, ANOVA showed a significant effect of TNF concentration (p<0.05 and p< 0.01 respectively). In C-F, ANOVA showed a significant effect of time and TNF dose (p<0.01 in every case). * = p<0.05, ** = p<0.01 compared to the initial time point by Dunnett test. Data are mean ± SEM from 3 experiments.