R-isovaline does not modulate GABA-evoked currents in transfected AtT20 cells.

<p><b>a</b>) Baseline subtracted IV curves for GABA (1 μM; n = 3) and co-application of R-isovaline with GABA (1 μM n = 3; 10 nM n = 6). <b>b</b>) Graph shows that R-isovaline does not alter the current evoked by a high concentration of GABA. Inset shows example currents at baseline (black), on application of GABA (1 μM; dark grey) and on co-application of 1 μM GABA with 250 μM R-isovaline (light grey). <b>c</b>) Graph shows that R-isovaline does not alter the current when co-applied with a low concentration of GABA. Inset shows example currents at baseline (black), on application of 1 μM GABA (dark grey), and on co-application of 10 nM GABA with 250 μM R-isovaline (light grey). Data are expressed as mean ± SEM. * = p < 0.05, ** = p < 0.01.