Neighbor-joining tree of <i>E</i>. <i>bieneusi</i> ITS genotypes.

<p>Phylogenetic relationship of <i>E</i>. <i>bieneusi</i> ITS nucleotide sequences of this study and other genotypes previously deposited in GenBank, as inferred by a neighbor-joining analysis (software Mega 5, <a href="http://www.megasoftware.net/" target="_blank">http://www.megasoftware.net/</a>) based on genetic distances calculated using the Kimura two-parameter model. The ITS tree was rooted with GenBank sequence DQ885585. Bootstrap values greater than 50% from 1,000 are shown on nodes. Each sequence from GenBank is identified by the accession number, host origin, and the genotype designation. The group terminology for the clusters is based on the works of Thellier and Breton [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref021" target="_blank">21</a>], Li et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref009" target="_blank">9</a>], and Karim et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117991#pone.0117991.ref003" target="_blank">3</a>]. Two unique sequences of new genotype CM18 and known genotype CM4 in this study are designated as group 9 sequences. Known and new genotypes identified in this study are indicated by open and filled triangles, respectively.