Influenza virus leads to enhanced CEACAM6 mRNA level and protein expression.

<p>Caco-2 cells were infected with PR8 (0.8 MOI) and harvested at different times p.i. Samples were used for mRNA or for protein extraction, as described in Methods. A: real-time PCR of CEACAM6 mRNA levels in infected cells harvested at 4, 6, 18 and 24 hrs p.i. Levels of mRNA are expressed as the ratio between CEACAM6 normalized for β-actin and GAPDH. B left panel: a rapresentative western blot analysis of CEACAM6 expression at different times 4, 6 and 24 hrs p.i. As controls, mock-infected cells were recovered at 4 and 24 hrs p.i. Samples were lysed, run onto SDS-PAGE, blotted and immunostained with anti-CEACAM6 Abs. Loading control was Actin. B right panel: densitometric analysis expressed as the ratio of CEACAM6 normalized for actin is reported. The values of western blot signals were obtained by densitometric analysis of control at 4 and 24 hrs respect to infected cell at 4, 6 and 24. Results are shown for the three representative experiments (*P<0.05, **P<0.01).