RAGE blockade inhibits calgranulin (hS100A8, hS100A9 or hS100A12) induced THP-1 cell migration but does not impact migration of all S100s.

<p>Migration indices for THP-1 cells in response to hS100A8 (A), hS100A9 (B), and hS100A12 (C). Effects of anti-RAGE, anti-hTLR4 Abs and isotype control Abs on hS100A8 (D), hS100A9 (E) and hS100A12 (F) induced THP-1 cell migration. Serial dilution of anti-RAGE, anti-TLR4 or isotype-matched control Ab were incubated with THP-1 cells in the upper wells of the chemotaxis chamber, and optimal amounts of hS100A8 (1 ng/ml), hS100A9 (1 ng/ml) and hS100A12 (100 ng/ml) were added in the lower wells. Percentage inhibition is relative to no Ab treatment. One representative of three independent experiments is shown (mean ±SD of triplicate wells) (G) The effects of a fixed 10 μg/ml dose of anti-RAGE, anti-hTLR4 and control Abs on THP-1 cell migration mediated by other S100s and MCP1. The S100s were used at their optimal concentrations (indicated on graphs). The maximal chemotactic indexes were S100A1 (2.0), S100A4 (2.9), S100A6 (2.9), S100A7 (3.7), S100A8/A9 (3.1), S100A10 (1.9), S100A14 (2.7), S100A16 (2.4), S100P (3.5), S100B (3.5) and MCP1 (9.5). Percentage inhibition is relative to no Ab treatment. Mean inhibition ± SD for one of two independent experiments is shown. Unpaired T-test was used to determine if the anti-RAGE or anti-hTLR4 Ab treatments were significantly different from the isotype control Ab (*P<0.05, **P<0.01, ***P<0.001).