Human S100A9 induced migration of leukocyte populations is inhibited by anti-RAGE Ab but not anti-TLR4 Ab.

<p>Chemotactic responses to S100A9 of human leukocyte populations were examined at the optimal concentration (1 ng/ml). The effects of TLR4 and RAGE blockade on S100A9-mediated migration of granulocytes (A), monocytes (B), and lymphocytes (C), with 10 μg/ml of anti-RAGE, anti-hTLR4 Ab and isotype control Abs. Data shown is mean±SD of triplicate samples from a representative of three independent experiments. Unpaired T-test was used to determine if the anti-RAGE or anti-TLR4 treatments were significantly different from the isotype control Ab (*P<0.05, **P<0.01, ***P<0.001).