The SATB1-overexpressing cells and SATB1-knockdown cells are established.

<p>The SATB1 expression of BIU-87 cells and T24 cells transfected with pcDNA3.1-SATB1 and pGenesil2-SATB1-shRNA were examined by qRT-PCR and western blot (A and B; **P < 0.001). Non-transfected BIU-87 cells were used as control group. T24 cells treated with pGenesil2 control vector which does not target any specific gene were used as the control groups. Immunofluorescence analysis was performed to detect the SATB1staining in each cell groups. Immunofluorescence images at 200×magnification (C and D). Error bars indicate s.e.m., n = 3 experiments.