Anti-inflammatory effect of GS12021 in RAW 264.7 cells.

<p>(A) Concentration-dependent effect of GS12021 on expression of iNOS and COX-2. Representative western blot images and quantification data are shown. Cells were pretreated with the indicated concentrations of GS12021 1 h before LPS treatment (10 ng/mL) for 24 h. (B) Nitrite production in cells from (A). (C) mRNA expression of inflammatory genes/cytokines. Cells were treated with agents for 6 h and RT-qPCR was performed. (D) Cytokine levels in supernatant media from cells treated with LPS ± GS12021 for 6 h (TNFα) or 24 h (IL-6 and MCP-1). All the experiments were repeated at least three times and the data are provided as mean ± SEM values. ** <i>P</i> < 0.01 <i>versus</i> CON; ^#<i>P</i> < 0.05, ^##<i>P</i> <0.01 <i>versus</i> LPS treatment alone.