Anti-inflammatory effect of GS12021 was preserved in SIRT1 knockout (KO) macrophages.

<p>(A) SIRT1 expression in peritoneal macrophages obtained from wild-type (WT) or myeloid-specific SIRT1 KO mice. Peritoneal macrophages were isolated from WT or SIRT1 KO mice as described in the Materials and Methods section. (B) mRNA levels of inflammatory genes measured by RT-qPCR in macrophages from WT or SIRT1 KO mice. Cells were pretreated with GS12021 for 1 h, and then treated with LPS (10 ng/mL) for 6 h. ** <i>P</i> < 0.01 <i>versus</i> CON, # <i>P</i><0.05 ## <i>P</i><0.01 <i>versus</i> LPS alone.