Analysis of the prevalence of cells undergoing apoptosis or cell division reveals that D_H altered B cells are less likely to undergo programmed cell death, but more likely to be quiescent.

<p><b>(A)</b><i>Percentage of developing Hardy Fractions B to E B cells in late stage apoptosis in the bone marrow</i>. A total of 14 WT BALB/c, 11 ΔD-DFL and 14 ΔD-iD mice were examined. Late apoptotic cells co-expressed PI and Annexin V. <b>(B)</b><i>Percentage of developing Hardy Fractions B to E B cells in G0 (quiescent cells)</i>, <i>S (actively synthesizing DNA)</i>, <i>G2-M (mitosis) and G1 (post-mitotic cells) as assessed by differential straining for BrdU and 7AAD</i>. A total of 6 WT, 11 ΔD-DFL and 10 ΔD-iD BALB/c mice were studied. <b>(C)</b><i>Percentage of maturing or mature B cell subsets in late stage apoptosis in the spleen or bone marrow</i>. T1, T2, MZ and FO cells were studied in the spleen, Hardy Fraction F was studied in the bone marrow. A total of 10 WT, 11 ΔD-DFL and 14 ΔD-iD BALB/c mice were studied. <b>(D)</b><i>Percentage of maturing or mature B cell subsets in G0</i>, <i>S</i>, <i>G2-M and G1 as assessed by differential straining for BrdU and 7AAD</i>. A total of 6 WT, 8 ΔD-DFL and 5ΔD-iD BALB/c mice were studied. The Standard error of the mean is presented as bars above each column. Significance values are listed as ‘*’, p ≤ 0.05; ‘**’, p ≤ 0.01; ‘***’, p < 0.001; and ‘****’, p < 0.0001.