Distribution of arginine in CDR-H3 as a function of D_H sequence.

<p><b>A</b>) Germline sequences of D_H genes and translation in reading frames (RF) 1–3. DFL16.1 RF1 encodes mostly neutral amino acids (green) while iD RF1 encodes mostly charged amino acids (red). In both genes, RF2 encodes hydrophobic amino acids (blue) and RF3 contains stop codons (*). <b>B</b>) Percentage CDR-H3 loop sequences containing arginines in Kabat position 95–98, 100–100a, or both. <b>C</b>) Distribution of arginines per CDR-H3 sequence in 8 week bone marrow immature B cells as a function of D_H content and the presence or absence of TdT. The sequences from TdT-sufficient ΔD-DFL, WT and ΔD-iD mice, and TdT deficient WT mice were previously reported, but reanalyzed for this work [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118171#pone.0118171.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118171#pone.0118171.ref034" target="_blank">34</a>]. The inner circle contains the total number of sequences examined.