2FF inhibits NF-ĸB activation and adhesion molecule expression in livers of sickle mice.

<p>After 7 days administration of water or 100 mM 2FF in drinking water and 4 hours after infusion of stroma-free Hb, livers from NY1DD sickle mice were removed and flash frozen. NF-kB phospho- and total-p65 were measured by western blot using liver nuclear extracts. Nuclear NF-ĸB phospho-p65 is a marker of NF-ĸB activation. VCAM-1, E-selectin and GAPDH were measured by western blot using liver microsomes.