Intestinal IgA responses in mice primed p.o. with soluble Ova, NP-Ova or PBS.

<p>Mice were administered PBS (control), soluble Ova, or 20 nm NP-Ova p.o. at day 0, 3, 6 and 8, then s.c. boosted with 300 μg Ova+CFA at day 28 (arrow). Fecal extracts were assayed for Ova-specific IgA using ELISA assay. Ova-specific IgA titers are expressed as log₁₀ titer values, with the titer being the highest dilution showing an absorbance value twice that of the background. Data collected from 10 mice per group (2 separate experiments) are expressed as the mean ± SD of the mean. Group means were separated using Tukey’s multiple comparison procedure and were declared significantly different at p<0.05 (*) and p<0.01 (***).