Sensitivity of mononuclear phagocytes in <i>L.donovani</i> infected mice to RTKi treatment.

<p>CD11c⁺MHCII⁺ MPs in d28 <i>L. donovani</i> infected mice were distinguished on the basis of F4/80 and CD11b expression, forward/side scatter profile and morphology into: (A) large CD11c⁺MHCII⁺F4/80^hiCD11b^lo (F4/80^hiCD11b^lo) cells with macrophage-like morphology, of which ∼80% harbored intracellular parasites; (B) slightly smaller CD11c⁺MHCII⁺F4/80^loCD11b^hi (F4/80^loCD11b^hi) cells with classic macrophage morphology, of which <5% harbored parasites; (C) much smaller CD11c⁺MHCII⁺F4/80^loCD11b^lo (F4/80^loCD11b^lo) cells with dendritic cell-like morphology and no observable parasites. Representative dot plots show pre-sorted populations with ellipsoid sort gates based on F4/80 and CD11b expression. Scale bar in micrographs = 10microns. The frequency and absolute numbers of each population is given in the right hand panels in naïve mice, infected mice and infected mice treated orally with sunitinib (Sm) for 7 days. P values = * <0.05, ** <0.008, ***<0.001, ns = not significant.