<p>Cells were incubated with either rabbit phospho-Ser40-TH plus mouse proteasome antibodies (upper panel) or rabbit phospho-Ser19-TH plus mouse proteasome antibodies (lower panel) followed by incubation with an anti-rabbit antibody coupled to Alexa 488 and anti-mouse coupled to AlexaFluor 594. See text for details. Scale bar = 5 μm. Three separate cell culture preparations were performed and 10 different images were taken and analyzed for each experiment. One representative image is presented.</p
<p>A549 cells were fixed with 4% para-formaldehyde and stained with a combination of a mouse anti Ep...
<p>Confocal immunofluorescence analysis of PML and 11Sβ were performed on U373MG cells stably expres...
<p>Cells cultured in DM at 37°C for 2 days were fixed and processed for confocal double-labelled ind...
<p>Cells were incubated with either mouse anti-TH plus rabbit anti-ubiquitin antibodies (upper panel...
<p>C2C12 cells were transfected with plasmids expressing the fluorescent proteins as indicated and d...
<p>HeLa cells were grown on glass cover slips and transfected with GFP-NPM1. After 24 hours, cells w...
<p>Pho1 specific monoclonal antibody H7c-crosslinked Protein A Sepharose CL-4B beads were used to pu...
anti-mouse IgM μ-chain-specific antibody was used as a secondary antibody for the Phospho-Ser-5 Pol ...
<p>Cells were cultured in DM at 37°C for 2 days, and subsequently, at 18°C for 2 h. After that, cell...
<p>THP-1 cells were treated with PMA for 72 h as previously, and then incubated with IgG-opsonised S...
<p>GFP-MAL2/HOG cells cultured in DM at 37°C for 2 days were fixed and processed for confocal triple...
<p>Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation...
The PA28, or 11S regulatory complex, stimulates the peptidase activities of the 20S proteasome. Mono...
<p>(A) Binding of HAMLET to 20S proteasome subunits. 20S proteasomes were denatured, separated by SD...
<p>(A) AP-3 was distributed in a punctate fashion in the cytoplasm of the RBL-2H3 cells. Double labe...
<p>A549 cells were fixed with 4% para-formaldehyde and stained with a combination of a mouse anti Ep...
<p>Confocal immunofluorescence analysis of PML and 11Sβ were performed on U373MG cells stably expres...
<p>Cells cultured in DM at 37°C for 2 days were fixed and processed for confocal double-labelled ind...
<p>Cells were incubated with either mouse anti-TH plus rabbit anti-ubiquitin antibodies (upper panel...
<p>C2C12 cells were transfected with plasmids expressing the fluorescent proteins as indicated and d...
<p>HeLa cells were grown on glass cover slips and transfected with GFP-NPM1. After 24 hours, cells w...
<p>Pho1 specific monoclonal antibody H7c-crosslinked Protein A Sepharose CL-4B beads were used to pu...
anti-mouse IgM μ-chain-specific antibody was used as a secondary antibody for the Phospho-Ser-5 Pol ...
<p>Cells were cultured in DM at 37°C for 2 days, and subsequently, at 18°C for 2 h. After that, cell...
<p>THP-1 cells were treated with PMA for 72 h as previously, and then incubated with IgG-opsonised S...
<p>GFP-MAL2/HOG cells cultured in DM at 37°C for 2 days were fixed and processed for confocal triple...
<p>Immunocytochemistry staining of cells in co-culture after liberation from MyDerm™ and cultivation...
The PA28, or 11S regulatory complex, stimulates the peptidase activities of the 20S proteasome. Mono...
<p>(A) Binding of HAMLET to 20S proteasome subunits. 20S proteasomes were denatured, separated by SD...
<p>(A) AP-3 was distributed in a punctate fashion in the cytoplasm of the RBL-2H3 cells. Double labe...
<p>A549 cells were fixed with 4% para-formaldehyde and stained with a combination of a mouse anti Ep...
<p>Confocal immunofluorescence analysis of PML and 11Sβ were performed on U373MG cells stably expres...
<p>Cells cultured in DM at 37°C for 2 days were fixed and processed for confocal double-labelled ind...