Quantitative RT-PCR analysis of expressions of the stress-related genes in <i>GhRAV1</i>-overexpression transgenic Arabidopsis under drought and salt stresses.

<p>Total RNA was isolated from the four-week-old transgenic plants and wild type grown under normal conditions and drought and NaCl stresses (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118056#sec002" target="_blank">Methods</a>). Transcript levels of <i>Arabidopsis RAB18, ABI1, ERD10, ERD15, KIN1, RD29A, RD29B</i> and <i>COR15a</i> genes in the transgenic plants and wild type were determined by quantitative RT-PCR, using <i>Arabidopsis ACTIN2</i> gene (<i>AtACT2</i>) as a quantification control. Mean values and standard errors were shown from three independent experiments with three biological replicates of plant materials. Independent t-tests demonstrated that there was significant difference (* P < 0.05;**P<0.01) in gene expression level between the transgenic lines and wild type. WT, wild type; L8, L21 and L23, three <i>GhRAV1</i> transgenic lines.