Phenotypic assay of the <i>GhRAV1</i>-overexpression transgenic <i>Arabidopsis</i> under high salinity stress.

<p>(<b>A</b>) Statistic analysis of seed germination rate of the <i>GhRAV1</i> transgenic lines and wild type on MS medium (control). (<b>B</b>) Statistic analysis of seed germination rate of <i>GhRAV1</i> transgenic lines and wild type on MS medium with 150mM NaCl. (<b>C</b>) Statistic analysis of seed germination rate of <i>GhRAV1</i> transgenic lines and wild type on MS medium with 200mM NaCl. (<b>D</b>) Statistic analysis of green seedling rate of <i>GhRAV1</i> transgenic lines and wild type grown on MS medium (control). (<b>E</b>) Statistic analysis of green seedling rate of <i>GhRAV1</i> transgenic lines and wild type grown on MS medium with 150mM NaCl. (<b>F</b>) Statistic analysis of green seedling rate of <i>GhRAV1</i> transgenic lines and wild type grown on MS medium with 200mM NaCl. (<b>G</b>) Phenotype of the <i>GhRAV1</i> transgenic plants and wild type grown in soil under normal conditions. (<b>H</b>) Phenotype of the <i>GhRAV1</i> transgenic plants and wild type grown in soil after watered with 150mM NaCl for one week. (<b>I</b>) Assay of chlorophyll content in leaves of the transgenic plants and wild type. 0.1g leaves were used to extract chlorophyll in a spectrophotometer (TU-1901) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118056#sec002" target="_blank">Methods</a>). (<b>J</b>) Assay of maximum photosynthesis efficiency (Fv/Fw) in leaves of the transgenic plants and wild type. Mean values and standard errors were shown from three independent experiments with three biological replicates of plant materials. Independent t-tests demonstrated that there was (very) significant difference (* P < 0.05 or ** P < 0.01) between the transgenic lines and wild type under NaCl stress (n > 200). Ca, chlorophyll a; Cb, chlorophyll b. WT, wild type; L8, L21 and L23, three transgenic lines.