<p><b>(A)</b> Immunofluorescence staining of F-actin in osteoblasts cultured on 1% BSA, 2 μg/ml of CTGF or fibronectin coated slides for 8 hours at 37°C. Scale bar = 50 μm. <b>(B)</b> Cell spreading area of osteoblasts cultured on BSA, CTGF or fibronectin for 8 hours and stained for actin were measured by ImageJ; n = 50. **p<0.01; ***p<0.001. <b>(C)</b> Western blot analysis of active Rac1, total Rac1 and actin to study Rac1 activation levels. Osteoblasts were cultured for 2 hours on uncoated plates or plates coated with BSA, CTGF or fibronectin. Abbreviations: fibronectin (FN) and negative control (Cont). Experiments were repeated three times with similar results.</p
Reducing the time required for initial integration of bone-contacting implants with host tissues wou...
<p>(<b>A</b>) Active Rac1 was immunoprecipitated from lysates derived from NIH 3T3 cells that had be...
<p>Osteoclasts were incubated for 5 hours in MEM/BSA with M-CSF (50 ng/ml), RANKL (30 ng/ml) and IL-...
<p><b>(A)</b> Adhesion assay to analyze osteoblast adhesion to various concentrations of recombinant...
<p><b>(A)</b> Alkaline Phosphatase (ALP) staining of osteoblasts cultured on 2 μg/ml CTGF or 1% BSA ...
<p>ROCK inhibition disrupts stressfibre formation in osteoblasts on both PT surfaces. Cells were sta...
<p><b>(A)</b> ChIP assay performed on osteoblasts cultured on CTGF coated, BSA coated or uncoated pl...
<p><b>A.</b> To compare the relative levels of ROCK activation, depending on the amount of precoated...
<p>A, Cells were transfected with pcDNA (1 µg) or RacN17 (0.5 and 1 µg) for 24 h and then stimulated...
<p>They were then placed into chondrocyte differentiation media (Stem Cell Technology) with or witho...
International audienceThe effects of mechanical strains on cellular activities were assessed in an i...
<p><b>(A)</b> RA synovial fibroblasts were cultured with 20 ng/ml of VEGF for 72 h and RANKL product...
Osteoclasts are multinucleated cells that resorb bone and carry out their function via a dynamic act...
Cell shape and regulation of biological processes such as proliferation and differentiation are to a...
Pre-osteoblast adhesion and interaction with extracellular matrix (ECM) proteins through integrin re...
Reducing the time required for initial integration of bone-contacting implants with host tissues wou...
<p>(<b>A</b>) Active Rac1 was immunoprecipitated from lysates derived from NIH 3T3 cells that had be...
<p>Osteoclasts were incubated for 5 hours in MEM/BSA with M-CSF (50 ng/ml), RANKL (30 ng/ml) and IL-...
<p><b>(A)</b> Adhesion assay to analyze osteoblast adhesion to various concentrations of recombinant...
<p><b>(A)</b> Alkaline Phosphatase (ALP) staining of osteoblasts cultured on 2 μg/ml CTGF or 1% BSA ...
<p>ROCK inhibition disrupts stressfibre formation in osteoblasts on both PT surfaces. Cells were sta...
<p><b>(A)</b> ChIP assay performed on osteoblasts cultured on CTGF coated, BSA coated or uncoated pl...
<p><b>A.</b> To compare the relative levels of ROCK activation, depending on the amount of precoated...
<p>A, Cells were transfected with pcDNA (1 µg) or RacN17 (0.5 and 1 µg) for 24 h and then stimulated...
<p>They were then placed into chondrocyte differentiation media (Stem Cell Technology) with or witho...
International audienceThe effects of mechanical strains on cellular activities were assessed in an i...
<p><b>(A)</b> RA synovial fibroblasts were cultured with 20 ng/ml of VEGF for 72 h and RANKL product...
Osteoclasts are multinucleated cells that resorb bone and carry out their function via a dynamic act...
Cell shape and regulation of biological processes such as proliferation and differentiation are to a...
Pre-osteoblast adhesion and interaction with extracellular matrix (ECM) proteins through integrin re...
Reducing the time required for initial integration of bone-contacting implants with host tissues wou...
<p>(<b>A</b>) Active Rac1 was immunoprecipitated from lysates derived from NIH 3T3 cells that had be...
<p>Osteoclasts were incubated for 5 hours in MEM/BSA with M-CSF (50 ng/ml), RANKL (30 ng/ml) and IL-...