CTB-INS vaccination of monocytes does not interfere with dendritic cell differentiation.

<p>Flow cytometric analysis of vaccinated monocyte differentiation into dendritic cells. <b>(A)</b> The CD14 monocyte marker becomes progressively less positive (number inside the box (gate), as DC differentiation proceeds in the presence of the CTB-INS fusion protein. <b>(B)</b> The HLA-DR marker indicative of antigen presenting cell DC morphogenesis increases as dendritic cell differentiation progresses in the presence of CTB-INS. <b>(C)</b> The co-stimulatory factor marker for DC maturation CD86 is observed to decrease with time in vaccinated DCs in comparison with background levels. <b>(D)</b> With time after inoculation with CTB-INS, the dendritic cell marker CD83 indicative of DC maturation decreases in comparison with unvaccinated DC background levels.