Common factors of antimonial resistance in different species of <i>Leishmania</i>.

<p><b>A</b>. Levels of <i>MRPA</i> mRNA: Total RNA was isolated from promastigotes of different species and <i>MRPA</i> mRNA expression levels were estimated using qPCR. Relative (with respect to <i>L</i>. <i>donovani</i>) MRPA expression levels were calculated using 2^-ΔΔCt method. Data were expressed as mean ± SD of three independent experiments in triplicate. <b>B</b>. Levels of total non-protein thiols: Promastigotes from different species of <i>Leishmania</i> were harvested and proteins were precipitated using tricloroacetic acid. Total non-protein thiols were estimated using dithionitrobenzoic acid. Data were expressed as mean ± SE of three independent experiments in triplicate. <b>C</b>. Levels of <i>AQP1</i> mRNA: Total RNA was isolated from promastigotes of different species and <i>AQP1</i> mRNA expression levels were estimated using qPCR. Relative (with respect to <i>L</i>. <i>donovani</i>) AQP1 expression levels were calculated using 2^-ΔΔCt method. Data were expressed as mean ± SD of three independent experiments in triplicate.