Activation status of spleen cells from mice immunized with rSm29 or rSm22.6, or inoculated with saline.

<p>One week after the last immunization, spleen cells from IT/Saline, IT/rSm22.6, IT/rSm29, rSm22.6 and rSm29 groups were labeled to evaluate the frequency of activated lymphocytes, and were acquired in a flow cytometer. Data analysis was carried out as follows (A): within singlet cells/lymphocyte region, cells expressing CD4, CD8 or CD19 molecules were selected and the expression of CD25 and CD69 activation molecules were evaluated in TCD4⁺ or TCD8⁺ cells. B-cells activation status was evaluated in CD19+ cells expressing the activation marker CD86. (B): Bars represent the median with interquartile range of the percentage of cells expressing the activation molecules. Statistically significant difference is pointed in the graphic. Statistical analyses were performed by the Mann-Whitney test.