The run length of transport by several kinesins is shown.

<p>(a) shows the run length of one, two, and three kinesins for [ATP] = 2 mM. (b) shows an example of HLB when a resisting load of 12 pN acts on the cargo. (b1) The leading kinesin is stationary while waiting for ATP. During this long interval, another kinesin binds to the MT. (b2) It is likely that the distance between the newly bound kinesin and the cargo is less than the length of the cargo linker. Thus, the newly bound kinesin does not have a load. Consequently, the lagging kinesin walks toward the leading one (the anchor) with high velocity. (b3) The two kinesins cooperate to transport their cargo against the large load. (b4) One of the kinesins unbinds and the other kinesin acts as an anchor again.