Transcript expression levels of Ca^<b>2+</b>-related signaling molecules.

<p>Quantitative RT-PCR was performed on B cells from 11 healthy donors and 50 representative CLL patients (Binet Stage A, <i>n</i> = 30; Binet Stage B/C, <i>n</i> = 20; five samples with dysfunctional <i>TP53</i>). Expression values indicate the mean value obtained for transcripts in CLL cells related to the mean value obtained for the same transcripts in normal B lymphocytes. Data were analyzed using the comparative threshold cycle method. The amount of cDNA measured is normalized to the endogenous references <i>GUSB</i> or <i>ABL</i> (see <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.1001796#sec010" target="_blank">Methods</a> for gene details). <i>PLCG1</i> is given in bold.</p><p>Transcript expression levels of Ca^<b>2+</b>-related signaling molecules.